- Advantage And Disadvantage Of The Serial Dilution Agar Plate Procedure Chart
- Advantages Of Serial Dilution-agar Plate Procedure
- Advantage And Disadvantage Of The Serial Dilution Agar Plate Procedure Instructions
Spread plate technique is the method of isolation and enumeration of microorganisms in a mixed culture and distributing it evenly. The technique makes it easier to quantify bacteria in a solution.
May 22, 2019 What Are Some Advantages And Disadvantages Of The Serial Dilution Agar Plate Technique; The agar dilution procedure is a technique approved by the Clinical and Laboratory Standards Institute (CLSI), for antimicrobial susceptibility testing of Campylobacter spp. Advantages And Disadvantages Of The Serial Dilution Agar Plate Technique. 10/8/2019 The Quadrajet (Q-Jet) carburetor is better on a street/daily driven vehicle for a couple reasons. One is that the primary throttle bores are pretty small, so at part throttle, like most driving, you won't be using the entire carb, and hence you'll use less gas. As the dilutions progress, you will reach a point where a reasonable number of colonies can be counted and multiplied by the dilution factor to get concentration. Disadvantages- does not separate bacteria like a streak plate and you have to work with liquid agar. Watsapp for pc.
Advantage And Disadvantage Of The Serial Dilution Agar Plate Procedure Chart
Principle of Spread Plate Technique
The spread plate technique involves using a sterilized spreader with a smooth surface made of metal or glass to apply a small amount of bacteria suspended in a solution over a plate. The plate needs to be dry and at room temperature so that the agar can absorb the bacteria more readily. A successful spread plate will have a countable number of isolated bacterial colonies evenly distributed on the plate.
Procedure of Spread Plate Technique
- Make a dilution series from a sample.
- Pipette out 0.1 ml from the appropriate desired dilution series onto the center of the surface of an agar plate.
- Dip the L-shaped glass spreader into alcohol.
- Flame the glass spreader (hockey stick) over a Bunsen burner.
- Spread the sample evenly over the surface of agar using the sterile glass spreader, carefully rotating the Petridish underneath at the same time.
- Incubate the plate at 37째C for 24 hours.
- Calculate the CFU value of the sample. Once you count the colonies, multiply by the appropriate dilution factor to determine the number of CFU/mL in the original sample.
Uses of Spread Plate Technique
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- It is used for viable plate counts, in which the total number of colony forming units on a single plate is enumerated.
- It is used to calculate the concentration of cells in the tube from which the sample was plated.
- Spread plating is routinely used in enrichment, selection, and screening experiments.
Limitations of Spread Plate Technique
- Strick aerobes are favored while microaerophilic tends to glow slower.
- Crowding of the colonies makes the enumeration difficult.
Advantages Of Serial Dilution-agar Plate Procedure
References
Advantage And Disadvantage Of The Serial Dilution Agar Plate Procedure Instructions
- M. J. Pelczar
- Mackie and McCartney. Practical Medical Microbiology
- Bailey & Scott’s Diagnostic Microbiology.
- Practical Microbiology. Pradeep Kumar Sharma.